CSIR-IGIB’s CRISPR genome editing system: Raising the bar with 1,300-fold higher specificity, strong on-target editing
In a detailed interview to the Science Chronicle, Dr. Sundaram Acharya formerly with CSIR-IGIB and currently a JSPS postdoctoral fellow at the University of Tokyo, and co-inventor of enFnCas9, explains how and why the indigenously developed CRISPR genome editing system using the enhanced FnCas9 (enFnCas9) variants fare better than the existing Cas9 tool — about twice the DNA cleavage speed, a nearly 3.5-fold broader target range in the human genome, and much stronger editing activity with excellent genome-wide specificity
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